| |
|
|
|
Molecular Biology Services
Molecular biology services encompass a wide range of laboratory techniques and analyses focused on studying the molecular mechanisms underlying biological processes. Here are some common molecular biology services offered by diagnostic centers and research laboratories:
DNA sequencing services involve determining the precise order of nucleotides in a DNA molecule. This technique is essential for genetic analysis, genome sequencing, mutation detection, and identifying genetic variations associated with diseases.
PCR services involve amplifying specific DNA sequences from a sample, enabling the detection and quantification of target DNA or RNA molecules. PCR is widely used in diagnostics, gene expression analysis, infectious disease detection, and genetic testing.
Gene expression analysis services measure the levels of messenger RNA (mRNA) or protein expression in cells or tissues. Techniques such as reverse transcription PCR (RT-PCR), quantitative PCR (qPCR), microarrays, and RNA sequencing are used to study gene expression patterns and regulatory mechanisms.
NGS services involve high-throughput DNA or RNA sequencing, enabling comprehensive analysis of entire genomes, transcriptomes, or targeted genomic regions. NGS is used for genomic research, personalized medicine, cancer genomics, and identifying genetic mutations associated with diseases.
Genotyping services determine genetic variations, including single nucleotide polymorphisms (SNPs), insertions, deletions, and copy number variations (CNVs). These services are used for population genetics studies, disease association studies, pharmacogenomics, and forensic DNA analysis.
Plasmid DNA preparation services involve isolating, purifying, and quantifying plasmid DNA from bacterial cultures. Plasmid DNA is used in various molecular biology applications, including cloning, gene expression studies, and recombinant protein production.
RNAi services utilize synthetic small interfering RNAs (siRNAs) or short hairpin RNAs (shRNAs) to silence gene expression in cells or organisms. RNAi is used for functional genomics studies, target validation, and therapeutic applications.
Protein analysis services involve studying protein structure, function, and interactions using techniques such as western blotting, enzyme assays, protein purification, and mass spectrometry. These services are essential for understanding protein signaling pathways, protein-protein interactions, and protein biomarker discovery.
CRISPR/Cas9 genome editing services utilize the CRISPR-Cas9 system to introduce precise genetic modifications in cells or organisms. CRISPR/Cas9 is used for gene knockout, gene knock-in, gene editing, and functional genomics studies.
|
|
|
|
|